WSISPDR Weakly Supervised Cell Instance Segmentation by Propagating from Detection Response-卡核

Weakly Supervised Cell Instance Segmentation
by Propagating from Detection Response

by Kazuya Nishimura, Ker Dai Fei Elmer, Ryoma Bise

Prerequisites

python >= 3.6
ubuntu 18.04
CPU or GPU(NVIDIA Driver >= 430)
matlab

Installation

Python setting

Conda user

conda env create -f=requirement.yml
conda activate pytorch

Docker user

docker build ./docker
sh run_docker.sh

Graph-cut installation

Graph-cut setting

We use following code.

https://jp.mathworks.com/matlabcentral/fileexchange/38555-kernel-graph-cut-image-segmentation

mkdir graphcut 
cd graphcut
wget http://www.wisdom.weizmann.ac.il/~bagon/matlab_code/GCmex1.9.tar.gz
tar -zxvf GCmex1.9.tar.gz
matlab -nodesktop -nosplash -r 'compile_gc; exit'
cd ..

Demo

This demo is only one image’s demo.
If you want to apply this method to your dataset, you should prepare the likelihood map.

python main.py -g

Back propagate from each cell

Use cuda

python propagate_main.py -g

Use cpu

python detection_train.py

Optins:

-i :input path(str)

-o :output path(str)

-w :weight path want to load

-g :whether use CUDA

Graph-cut

matlab -nodesktop -nosplash -r 'graphcut; exit'

This is a sample code.

We don’t provide dataset.

If you want to apply your dataset, you should prepare the original image and point level annotation(cell centroid).
The attached text file (sample_cell_position.txt) contains a cell position(frame,x,y) as each row.
Prepare the same format text file for your dataset.

Generate likelyfood map

Set the variance to a value sufficiently larger than the target object.
The guided backpropagation depends on variance size.

python likelymapgen.py

Option:

-i :txt_file_path (str)

-o :output_path (str)

-w :width (int)

-h :height (int)

-g :gaussian variance size (int)

Train cell detection CNN

Use cuda

python detection_train.py -g

Use cpu

python detection_train.py

Optins:

-t :train path(str)

-v :validation path(str)

-w :save path of weight(str)

-g :whether use CUDA

-b :batch size (default is 16)

-e :epochs (default is 500)

-l :learning rate(default is 1e-3)

Predict cell detection

Use cuda

python detection_predict.py -g

Use cpu

python detection_predict.py

Optins:

-i :input path(str)

-o :output path(str)

-w :weight path want to load

-g :whether use CUDA

citation

If you find the code useful for your research, please cite:

@inproceedings{nishimura2019weakly,
  title={Weakly Supervised Cell Instance Segmentation by Propagating from Detection Response},
  author={Nishimura, Kazuya and Bise, Ryoma and others},
  booktitle={International Conference on Medical Image Computing and Computer-Assisted Intervention},
  pages={649--657},
  year={2019},
  organization={Springer}
}